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Cas9-pDBD target site algorithm for identifying sites and outputting ZFP sequences

Please upload the exon sequence file in fasta format w/line break: Click here for an example of input file

Or paste the sequences into the following text area in fasta format w/line break:


The input sequence should have ~25 bases flanking each side of the target region, as the program searches 50 base elements for target seq.

Enter the minimum score for the ZFP target site: maximum score is 8

Output to: Click here for an example of output file

   

Click here for more information on the algorithm, its scoring function and the output file
**We recommend targeting only GG PAMs with Cas9MT3; GG or AG PAMs with Cas9MT2**