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ZFN target site algorithm for identifying sites for selection using the Bacterial one hybrid system
This algorithm will also aid you in the design of libraries for the target sites using a combination of design and selection

Please upload the exon sequence file in fasta format w/line break: Click here for an example of input file

Or paste the sequences into the following text area in fasta format w/line break:


Enter the minimum number of purines per composite site:

Enter the minimum number of guanines per composite site:

Output to: Click here for an example of output file

   

Click here for a general protocol for zinc finger library construction in the omega-based B1H system

DNA/RNA Complementarity Convention

BaseNameBases RepresentedComplementary Base
AAdenineAT
TThymidineTA
UUridine(RNA only)U A
GGuanidineGC
CCytidineCG
YpYrimidineC TR
RpuRineA GY
SStrong(3Hbonds)G CS
WWeak(2Hbonds)A TW
KKetoT/U GM
MaMinoA CK
Bnot AC G TV
Dnot CA G TH
Hnot GA C TD
Vnot T/UA C GB
NUnknownA C G TN