Running OneStopRNAseq (OSR) with RNK input

If users have a gene rank-table, they can use this RNK entry to perform quick GSEA analysis with OSR. Below are the supported formats of rnk-table. Please click the example files to see the format.

Description of rnk-table format

Two different formats of rnk-table are supported

1. txt format

• Example file: example_rankfile.rnk.txt
• This is a plain xlsx file
• Must contain two columns separaed by tab
• Column 1 must be gene symbols
• Column 2 can be of any pre-ranked scores

2. Excel format

• Example file: example_rankfile_multiple_sheet.xlsx
• The xlsx file can have multiple sheets for creating multiple GSEA analysis and multi-bubble plot
• Each sheet must have a header row
• Each sheet must have two columns: “Gene_Symbol”, and “Number for ranking, e.g. LFC, p-value”
• The first cell in the first row will be used as the name displayed in the multi-bubble plot, e.g. "Comparison1" in the example file